ISOLATION OF CONCANAVALIN-A BY AFFINITY PRECIPITATION

被引：0

作者：

LARSSON, EL ^{[1
]}

MATTIASSON, B ^{[1
]}

机构：

[1] LUND UNIV,CTR CHEM,DEPT BIOTECHNOL,POB 124,S-22100 LUND,SWEDEN

来源：

BIOTECHNOLOGY TECHNIQUES | 1994年 / 8卷 / 01期

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Concanvalin A (Con A) was isolated from a crude extract of jack beans by affinity precipitation. p-Aminophenyl-alpha-D-glucopyranoside was coupled to the polymer Eudragit S-100. Affinity binding took place at pH 7.5 and precipitation was initiated by lowering pH to 5.2. The lectin was eluted by dissolving the polymer in the presence of 0.2 M methyl-alpha-D-mannopyranoside and then precipitating the polymer only. The polymer-ligand was recycled and used five times.

引用

页码：51 / 56

页数：6

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