REGULATION OF THE TNF-ALPHA RECEPTOR IN HUMAN OSTEOSARCOMA CELLS - ROLE OF MICROTUBULES AND OF PROTEIN-KINASE-C

被引:7
作者
GALEOTTI, T
BOSCOBOINIK, D
AZZI, A
机构
[1] UNIV BERN, INST BIOCHEM & MOLEK BIOL, BUHLSTR 28, CH-3012 BERN, SWITZERLAND
[2] UNIV CATTOLICA SACRO CUORE, IST PATOL GEN, I-00168 ROME, ITALY
关键词
D O I
10.1006/abbi.1993.1040
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The effect of the tumor promoter 4β-phorbol 12-myristate 13-acetate and of the phosphatases inhibitor okadaic acid on the binding of tumor necrosis factor-α (TNFα) to a human osteogenic sarcoma cell line (Saos-2) was investigated. Both substances prevented almost completely TNF binding to its receptors. The effect of 4β-phorbol 12-myristate 13-acetate was reversed by the protein kinase C inhibitors staurosporine and caiphostin C or by protein kinase C depletion. Vinblastine, under conditions causing full microtubule disassembly, produced only a 50% decrease of TNF binding. Viablastine plus PMA was additive in fully preventing TNF binding. It is suggested that the degree of binding of TNF-α to its receptors in Saos-2 cells is under the control of a microtubule-dependent and of a microtubule-independent regulatory pathway. © 1993 Academic Press, Inc.
引用
收藏
页码:287 / 292
页数:6
相关论文
共 31 条
[1]  
AGGARWAL BB, 1986, J BIOL CHEM, V261, P3652
[2]  
AGGARWAL BB, 1987, J BIOL CHEM, V262, P16450
[3]  
AGGARWAL BB, 1985, NATURE, V318, P65
[4]  
BAUD L, 1990, J IMMUNOL, V145, P556
[5]   IDENTITY OF TUMOR NECROSIS FACTOR AND THE MACROPHAGE-SECRETED FACTOR CACHECTIN [J].
BEUTLER, B ;
GREENWALD, D ;
HULMES, JD ;
CHANG, M ;
PAN, YCE ;
MATHISON, J ;
ULEVITCH, R ;
CERAMI, A .
NATURE, 1985, 316 (6028) :552-554
[6]   INHIBITION OF PROTEIN-KINASE-C BY CALPHOSTIN-C IS LIGHT-DEPENDENT [J].
BRUNS, RF ;
MILLER, FD ;
MERRIMAN, RL ;
HOWBERT, JJ ;
HEATH, WF ;
KOBAYASHI, E ;
TAKAHASHI, I ;
TAMAOKI, T ;
NAKANO, H .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 176 (01) :288-293
[7]   THE MOLECULAR ACTION OF TUMOR-NECROSIS-FACTOR-ALPHA [J].
CAMUSSI, G ;
ALBANO, E ;
TETTA, C ;
BUSSOLINO, F .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 202 (01) :3-14
[8]   ENDOTOXIN-INDUCED SERUM FACTOR THAT CAUSES NECROSIS OF TUMORS [J].
CARSWELL, EA ;
OLD, LJ ;
KASSEL, RL ;
GREEN, S ;
FIORE, N ;
WILLIAMSON, B .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (09) :3666-3670
[9]   OKADAIC ACID - A NEW PROBE FOR THE STUDY OF CELLULAR-REGULATION [J].
COHEN, P ;
HOLMES, CFB ;
TSUKITANI, Y .
TRENDS IN BIOCHEMICAL SCIENCES, 1990, 15 (03) :98-102
[10]  
DEMBIC Z, 1990, Cytokine, V2, P231, DOI 10.1016/1043-4666(90)90022-L