CRYSTAL-STRUCTURE OF A SRC-HOMOLOGY-3 (SH3) DOMAIN

被引：417

作者：

MUSACCHIO, A ^{[1
]}

NOBLE, M ^{[1
]}

PAUPTIT, R ^{[1
]}

WIERENGA, R ^{[1
]}

SARASTE, M ^{[1
]}

机构：

[1] EUROPEAN MOLEC BIOL LAB,MEYERHOFSTR 1,W-6900 HEIDELBERG,GERMANY

来源：

NATURE | 1992年 / 359卷 / 6398期

关键词：

D O I：

10.1038/359851a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

THE Src-homologous SH3 domain is a small domain present in a large number of proteins that are involved in signal transduction, such as the Src protein tyrosine kinase, or in membrane-cytoskeleton interactions, but the function of SH3 is still unknown (reviewed in refs 1-3). Here we report the three-dimensional structure at 1.8 angstrom resolution of the SH3 domain of the cytoskeletal protein spectrin expressed in Escherichia coli. The domain is a compact beta-barrel made of five antiparallel beta-strands. The amino acids that are conserved in the SH3 sequences are located close to each other on one side of the molecule. This surface is rich in aromatic and carboxylic amino acids, and is distal to the region of the molecule where the N and C termini reside and where SH3 inserts into the alpha-spectrin chain. We suggest that a protein ligand binds to this conserved surface of SH3.

引用

页码：851 / 855

页数：5

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