NONRADIOACTIVE LABELING OF OLIGONUCLEOTIDES INVITRO WITH THE HAPTEN DIGOXIGENIN BY TAILING WITH TERMINAL TRANSFERASE

被引：324
作者：
SCHMITZ, GG [1 ]
WALTER, T [1 ]
SEIBL, R [1 ]
KESSLER, C [1 ]
机构：
[1] BOEHRINGER MANNHEIM GMBH, BIOCHEM RES CTR, NONNENWALD 2, W-8122 PENZBERG, GERMANY
来源：
ANALYTICAL BIOCHEMISTRY | 1991年 / 192卷 / 01期
关键词：
D O I：
10.1016/0003-2697(91)90212-C
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A procedure for the nonradioactive labeling of oligonucleotides with the hapten digoxigenin (DIG) has been developed. The label is introduced by enzymatic tailing of the 3′-end. Two different modified nucleotides were applied. DIG-dUTP allows the synthesis of hapten-modified oligonucleotides with longer tails containing several DIG molecules, whereas the novel compound DIG-ddUTP leads to the addition of only a single DIG hapten. The efficiency of the labeling reactions with respect to variation of the different parameters was analyzed and data on application of labeled oligonucleotide probes to different blot formats are shown. © 1991.
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页码：222 / 231
页数：10
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