DEVELOPMENT AND VALIDATION OF A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY SYSTEM FOR THE ANALYSIS OF HOP BITTER ACIDS

被引：15
作者：
HERMANSLOKKERBOL, ACJ
VERPOORTE, R
机构：
[1] Leiden/Amsterdam Center for Drug Research, Division of Pharmacognosy, Gorlaeus Laboratories, 2300 RA Leiden
来源：
JOURNAL OF CHROMATOGRAPHY A | 1994年 / 669卷 / 1-2期
关键词：
D O I：
10.1016/0021-9673(94)80337-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An isocratic HPLC system is developed for the baseline separation of the six main hop bitter acids on a RP C,, column within 25 min. The eluent contains 0.05 M triethanolamine in methanol-water (65:35, v/v), the pH is set at 6.85 with phosphoric acid. The contribution of each of the components of the eluent on retention and resolution is investigated as well as the linearity for determination of the six main bitter acids.
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页码：65 / 73
页数：9
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