THIOL AND DISULFIDE DETERMINATION BY FREE ZONE CAPILLARY ELECTROPHORESIS

被引：30
作者：
CAPPIELLO, M
DELCORSO, A
CAMICI, M
MURA, U
机构：
[1] UNIV PISA, DIPARTIMENTO FISIOL & BIOCHIM, VIA S MARIA 55, I-56100 PISA, ITALY
[2] UNIV MODENA, FAC MED, IST CHIM BIOL, I-41100 MODENA, ITALY
来源：
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1993年 / 26卷 / 04期
关键词：
GLUTATHIONE; CYST(E)INE; CYST(E)AMINE; THIOL; DISULFIDE; CAPILLARY ELECTROPHORESIS;
D O I：
10.1016/0165-022X(93)90034-L
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A rapid, sensitive and simple method for the determination of reduced and oxidized glutathione, cysteine, cystine, cysteamine, cystamine and their respective mixed disulfides is described. The compounds were separated and identified in a single step by capillary zone electrophoresis. The method was used to follow the thiol-disulfide interconversion and to measure glutathione levels in lens extracts.
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页码：335 / 341
页数：7
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