PREPARATION OF PEPTIDE-CARRYING MICROSPHERES WITH BIOACTIVITY ON PLATELETS

被引：4
作者：
KASUYA, Y
FUJIMOTO, K
MIYAMOTO, M
JUJI, T
OTAKA, A
FUNAKOSHI, S
FUJII, N
KAWAGUCHI, H
机构：
[1] KEIO UNIV,FAC SCI & TECHNOL,3-14-1 HIYOSHI,KOHOKU KU,YOKOHAMA,KANAGAWA 223,JAPAN
[2] JAPANESE RED CROSS SOC,CTR CENT BLOOD,DEPT RES,SHIBUYA KU,TOKYO 150,JAPAN
[3] KYOTO UNIV,FAC PHARMACEUT SCI,SAKYO KU,KYOTO 606,JAPAN
来源：
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION | 1993年 / 4卷 / 04期
关键词：
MICROSPHERE; RGDS; PLATELET AGGREGATION; CELL ADHESION; BIOSPECIFIC ACTIVITY;
D O I：
10.1163/156856293X00050
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
A tetrapeptide RGDS (Arg-Gly-Asp-Ser), which is one of the active sites of cell adhesive proteins, was synthesized according to the solution method and covalently coupled onto monodisperse microspheres. The biospecific activity of the RGDS-carrying microspheres was investigated by measuring the stimulatory effect of the microspheres on platelets. Large and tightly packed platelet aggregation accompanied with drastic ATP release was induced by the RGDS-carrying microspheres. It was confirmed that the specific platelet aggregation was attributed to the activity of the immobilized RGDS by comparison with those induced by other microspheres.
引用
收藏
页码：369 / 380
页数：12
相关论文
共 27 条
  • [1] [Anonymous], 1984, PRACTICE PEPTIDE SYN
  • [2] [Anonymous], 1986, BASIC TECHNIQUES TRA
  • [3] BEER JH, 1992, BLOOD, V79, P117
  • [4] Bodanszky M., 1984, PRINCIPLES PEPTIDE S, DOI [10.1007/978-3-642-96835-8, DOI 10.1007/978-3-642-96835-8]
  • [5] DANILOV YN, 1986, EXP CELL RES, V182, P186
  • [6] HOLSEN H, 1966, ANAL BIOCHEM, V17, P456
  • [7] DESIGN AND SYNTHESIS OF BIOCOMPATIBLE POLYMERIC MATERIALS
    IMANISHI, Y
    ITO, Y
    LIU, LS
    KAJIHARA, M
    [J]. JOURNAL OF MACROMOLECULAR SCIENCE-CHEMISTRY, 1988, A25 (5-7): : 555 - 570
  • [8] ISHIKAWA Y, 1991, Journal of Biomaterials Science Polymer Edition, V2, P53, DOI 10.1163/156856291X00052
  • [9] FUNDAMENTAL-STUDY ON LATEX REAGENTS FOR AGGLUTINATION TESTS
    KAWAGUCHI, H
    SAKAMOTO, K
    OHTSUKA, Y
    OHTAKE, T
    SEKIGUCHI, H
    IRI, H
    [J]. BIOMATERIALS, 1989, 10 (04) : 225 - 229
  • [10] PREPARATION OF AMPHOTERIC LATEX BY MODIFICATION OF STYRENE-ACRYLAMIDE CO-POLYMER LATEX
    KAWAGUCHI, H
    HOSHINO, H
    OHTSUKA, Y
    [J]. JOURNAL OF APPLIED POLYMER SCIENCE, 1981, 26 (06) : 2015 - 2022
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