THE EFFECTS OF INDUCING AGENTS ON CYTOCHROME-P450 AND UDP-GLUCURONOSYLTRANSFERASE ACTIVITIES IN HUMAN HEPG2 HEPATOMA-CELLS

被引：71
作者：
DOOSTDAR, H
GRANT, MH
MELVIN, WT
WOLF, CR
BURKE, MD
机构：
[1] UNIV ABERDEEN MARISCHAL COLL, DEPT BIOMED SCI, ABERDEEN AB9 1AS, SCOTLAND
[2] UNIV ABERDEEN, DEPT MED & THERAPEUT, ABERDEEN AB9 1AS, SCOTLAND
[3] UNIV ABERDEEN, DEPT BIOCHEM, ABERDEEN AB9 1AS, SCOTLAND
[4] UNIV EDINBURGH, ICRF LAB, EDINBURGH EH8 9XD, SCOTLAND
来源：
BIOCHEMICAL PHARMACOLOGY | 1993年 / 46卷 / 04期
基金：
英国惠康基金;
关键词：
D O I：
10.1016/0006-2952(93)90548-B
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Selective induction in vitro of cytochrome P450-dependent mixed-function oxidase (MFO) and UDP-glucuronyltransferase (GT) activities was observed in the human HepG2 hepatoma cell line. 1,2-Benzanthracene (BA) induced MFO O-dealkylation activities for ethoxyresorufin, methoxyresorufin and benzyloxyresorufin, whereas phenobarbitone (PB) selectively induced pentoxyresorufin O-dealkylation and rifampicin (RIF) selectively induced benzyloxyresorufin O-dealkylation. Antibody inhibition experiments indicated that ethoxyresorufin and methoxyreSorufin O-dealkylations were catalysed mainly by the P450 1A subfamily in untreated and BA-induced HepG2 cells, that additional unidentified P450 forms were considerably involved in methoxyresorufin and benzyloxyresorufin O-dealkylations and that the P450 2B subfamily was partially responsible for pentoxyresorufin O-dealkylation in PB-induced cells. Bilirubin GT activity was induced by PB, BA, RIF and dexamethasone, but 1-naphthol, morphine and testosterone GT activities were not induced by any of these treatments.
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页码：629 / 635
页数：7
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