RAPID AND SIMPLE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF TRICYCLIC ANTIDEPRESSANTS FOR ROUTINE AND EMERGENCY SERUM ANALYSIS

被引：25

作者：

SEGATTI, MP ^{[1
]}

NISI, G ^{[1
]}

GROSSI, F ^{[1
]}

MANGIAROTTI, M ^{[1
]}

LUCARELLI, C ^{[1
]}

机构：

[1] IST SUPER SANITA,BIOCHIM CLIN LAB,I-00161 ROME,ITALY

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 536卷 / 1-2期

关键词：

D O I：

10.1016/S0021-9673(01)89265-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An isocratic reversed-phase high-performance liquid chromatographic procedure is presented for the simultaneous detection of desipramine, nortriptyline, imipramine, amitriptyline and clomipramine in serum. Drugs are extracted after sample alkalinization and separated from each other on an octyl reversed-phase with n-butylamine as mobile phase modifier. Detection is achieved at 254 nm. The recovery of tricyclic antidepressants (92-110%) has good precision, with a relative standard deviation of less than 5%. Being rapid and simple, the method is suitable for the emergency clinical laboratory.

引用

页码：319 / 325

页数：7

共 20 条

[1]

ASBERG M, 1978, COMMUN PSYCHOPHARMAC, V2, P381

[2]

BAILEY DN, 1976, CLIN CHEM, V22, P1697

[3] CLINICAL PHARMACOLOGY AND TOXICOLOGY OF ANTIDEPRESSANTS [J].

BIGGS, JT .

HOSPITAL PRACTICE, 1978, 13 (02) :79-84

[4] CHEMICAL IONIZATION MASS FRAGMENTOGRAPHIC MEASUREMENT OF DOTHIEPIN PLASMA-CONCENTRATIONS FOLLOWING A SINGLE ORAL DOSE IN MAN [J].

CRAMPTON, EL ;

GLASS, RC ;

MARCHANT, B ;

REES, JA .

JOURNAL OF CHROMATOGRAPHY, 1980, 183 (02) :141-148

[5]

DUGAS M, 1980, THER DRUG MONIT, V2, P307

[6] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SYSTEM FOR THE SEPARATION OF TRICYCLIC ANTIDEPRESSANT AND RELATED DRUGS USING ODS-HYPERSIL [J].

GILL, R ;

WANOGHO, SO .

JOURNAL OF CHROMATOGRAPHY, 1987, 391 (02) :461-464

[7]

HAVEN MC, 1987, CLIN CHEM, V33, P1472

[8]

HOLISTER LE, 1981, DRUGS, V22, P129

[9] RAPID, SENSITIVE GAS-LIQUID-CHROMATOGRAPHIC METHOD FOR DETERMINATION OF AMITRIPTYLINE AND NORTRIPTYLINE IN PLASMA USING A NITROGEN-SENSITIVE DETECTOR [J].

HUCKER, HB ;

STAUFFER, SC .

JOURNAL OF CHROMATOGRAPHY, 1977, 138 (02) :437-442

[10]

KOTEEL P, 1982, CLIN CHEM, V28, P462

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